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Updated: Feb 12, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures
Published on: January 13, 2021
Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI
Fabian Hertel1, Gary C H Mo2, Peter Dedecker1
1Department of Chemistry, University of Leuven, Heverlee, Belgium.
Abstract:
Few approaches are currently available that allow the detection of protein-protein interactions (PPIs) in super-resolution, and the observation of the assembly of protein complexes in living cells has been particularly challenging. We developed reconstituted fluorescence-based stochastic optical fluctuation imaging (refSOFI), which is based on bimolecular fluorescence complementation (BiFC) and SOFI, allowing us to detect protein complex assembly 30 min after the induction of complex formation. Here we describe how to use refSOFI to map the assembly of two proteins of interest into a complex within living cells at super-resolution.
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