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Expression of aquaporins mRNAs in patients with otitis media

Su Young Jung1, Sung Su Kim2, Young Il Kim3

  • 1a Department of Otorhinolaryngology, Head and Neck Surgery, School of Medicine , Kyung Hee University , Seoul , South Korea.

Acta Oto-Laryngologica
|April 3, 2018
PubMed
Abstract

Insights

Aquaporin (AQP) mRNA levels vary across different types of otitis media (OM). These AQP mRNA expressions correlate with specific clinical features and bacterial presence in OM patients.

Area of Science:

  • Otolaryngology
  • Molecular Biology
  • Genetics

Background:

  • Otitis media (OM) encompasses various middle ear inflammatory conditions.
  • Aquaporins (AQPs) are water channel proteins implicated in fluid balance and inflammation.
  • Understanding AQP expression in OM may reveal disease mechanisms.

Purpose of the Study:

  • To investigate the association between aquaporin (AQP) mRNA levels and clinical manifestations in different types of otitis media (OM).
  • To compare AQP mRNA expression patterns across chronic otitis media (COM), cholesteatomatous otitis media (choleOM), and otitis media with effusion (OME).

Main Methods:

  • Real-time PCR was used to quantify mRNA levels of eight AQP subtypes (AQP1-10, excluding AQP7, 9) in 163 OM patients.
  • Samples included inflammatory tissue, cholesteatoma matrix, and effusion fluid.
  • Statistical analyses correlated AQP mRNA levels with OM type, bacterial presence, and hearing loss.

Main Results:

  • All eight AQP mRNAs were detected in all OM types.
  • Elevated AQP2, 4, 6, 10 mRNA in OME and AQP1, 3, 4, 10 mRNA in choleOM compared to COM (p < .05).
  • Specific AQP mRNA levels (AQP1, 5, 10) differed significantly in relation to bacterial culture results across OM subtypes.

Conclusions:

  • Aquaporin (AQP) mRNA expression levels are significantly associated with the pathophysiology of different otitis media (OM) subtypes.
  • These findings suggest AQPs play a role in the distinct clinical presentations and disease progression of OM.
Keywords:
Aquaporincholesteatomachronic suppurative otitis mediamiddle earotitis mediaotitis media with effusion

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