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Updated: Feb 12, 2026

Using TgVtg1:mcherry Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds
Published on: August 8, 2020
In vivo effects of PCB-126 and genistein on vitellogenin expression in zebrafish
Patrizia Licata1, Roberta Tardugno2, Simona Pergolizzi3
1a Department of Veterinary Science, Veterinary Pharmacology and Toxicology , University of Messina , Messina , Italy.
Abstract:
In this study, the vitellogenin (Vtg) modulation by genistein and polychlorinated biphenyl-126 (PCB-126) exposure in zebrafishes has been investigated. Both PCB-126 and genistein have been identified as aquatic pollutants and can further increase estrogenicity of waterways. Vtg is egg yolk precursor protein release by the hepatocytes during vitellogenesis. This process occurs normally in the hepatocytes in response to the activation with the estrogens such as 17-β-estradiol. Our immunohistochemical findings showed a Vtg expression that increases at 12 h and at 72 h in the liver of treated fishes with both PCB-126 and genistein, individually and in combination. Furthermore, for the first time, also hepatic stellate cells (HSC) in the liver parenchyma were strongly positive for vitellogenin.
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