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Updated: Feb 12, 2026

Mating-based Overexpression Library Screening in Yeast
Published on: July 6, 2018
Variations in mating-type-like (MTL) loci direct PCR-based tracking of Zygosaccharomyces strains formed by mating
Tomoo Ogata1, Katsuaki Kuroki1, Koki Ito1
1Department of Biotechnology, Maebashi Institute of Technology.
Abstract:
Variations of chromosomal structures and nucleotide sequences around mating-type-like (MTL) loci among Zygosaccharomyces species have been reported. We have analyzed these differences in more detail and, on the basis of PCR- and next-generation sequencing data, we describe the MTL loci on chromosomes C and F for Z. rouxii type-strain NBRC1130, Z. rouxii NBRC0740 and Zygosaccharomyces sp. NBRC1876. We developed a mating strategy for Zygosaccharomyces sp. NBRC1876 and Z. rouxii NBRC0740, and found that the mated stains could be identified from parental strains on the basis of nucleotide sequence variations of the MTL loci. We further obtained evidence that Zygosaccharomyces sp. NBRC1876 is a natural interspecies hybrid between Z. rouxii and a related species.
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