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In-vitro biosynthesis of C18 neutral steroids in horse testes
S J Smith1, J E Cox, E Houghton
1Department of Veterinary Clinical Science, University of Liverpool, Leahurst, South Wirral, U.K.
Journal of Reproduction and Fertility. Supplement
|January 1, 1987
Abstract:
Deuterium, 14C- and 3H-labelled steroid substrates were incubated with minced testicular tissue from stallions of different ages. After extraction and separation of the neutral and phenolic fractions the metabolites were identified by gas chromatography-mass spectrometry. The presence of the expected C19 neutral and C18 phenolic steroids was confirmed. An isomer of 5(10)-oestrene-3,17-diol was also identified.