Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

65.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
65.4K
Plastic Deformations01:19

Plastic Deformations

477
Plastic deformation represents a fundamental concept in materials science, which explains the irreversible change in the shape of a material when it experiences stress beyond its elastic capability. This phenomenon is important in structural engineering, especially in designing and analyzing cantilever beams—structures that are securely fixed at one end and bear loads at the opposite end. When these beams are subjected to loads within their elastic range, they will return to their...
477
Plastic Deformations01:14

Plastic Deformations

477
It is essential to understand how structural members behave under plastic deformation when the bending stress exceeds the material's yield strength. This state of deformation permanently alters the shape of the member, in contrast to the linear elastic behavior observed before yielding. The strain at any point in the member is expressed in terms of maximum strain. Notably, the neutral axis, which coincides with the centroid during elastic bending, shifts away from the centroid under plastic...
477
Temperature Dependent Deformation01:12

Temperature Dependent Deformation

415
In a nonhomogeneous rod made up of steel and brass, restrained at both ends and subjected to a temperature change, several steps are involved in calculating the stress and compressive load. Due to the problem's static indeterminacy, one end support is disconnected, allowing the rod to experience the temperature change freely. Next, an unknown force is applied at the free end, triggering deformations in the rod's steel and brass portions. These deformations are then calculated and added...
415
Deformations in a Symmetric Member in Bending01:18

Deformations in a Symmetric Member in Bending

528
When analyzing the deformation of a symmetric prismatic member subjected to bending by equal and opposite couples, it becomes clear that as the member bends, the originally straight lines on its wider faces curve into circular arcs, with a constant radius centered at a point known as Point C. This phenomenon helps to understand the stress and strain distribution within the member more clearly.
When the member is segmented into tiny cubic elements, it is observed that the primary stress...
528
Deformation of Member under Multiple Loadings01:11

Deformation of Member under Multiple Loadings

491
When a rod is made of different materials or has various cross-sections, it must be divided into parts that meet the necessary conditions for determining the deformation. These parts are each characterized by their internal force, cross-sectional area, length, and modulus of elasticity. These parameters are then used to compute the deformation of the entire rod.
In the case of a member with a variable cross-section, the strain is not constant but depends on the position. The deformation of an...
491

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex differences in the long-term tolerability of BNT162b2 in children and adolescents.

Molecular and cellular pediatrics·2026
Same author

3D epithelial cell topology tunes signaling range to promote precise patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Risk factors for severe post-COVID condition in children, adolescents, and young adults.

European journal of pediatrics·2026
Same author

Cell viscosity influences haematogenous dissemination and metastatic extravasation of tumour cells.

Nature materials·2026
Same author

Molecular structure of the ESCRT-III-based archaeal CdvAB cell division machinery.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Activating PRKG1 Variant Enhances Smooth Muscle Cell Deformability to Cause Aortopathy.

JACC. Basic to translational science·2026
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.8K

Real-time fluorescence and deformability cytometry.

Philipp Rosendahl1, Katarzyna Plak1,2, Angela Jacobi1

  • 1Biotechnology Center, Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Dresden, Germany.

Nature Methods
|April 3, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces real-time fluorescence and deformability cytometry (RT-FDC), a novel instrument combining cell mechanics and molecular identification. This approach enhances specificity in label-free cell analysis, enabling new research possibilities.

More Related Videos

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.7K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

7.0K

Related Experiment Videos

Last Updated: Feb 12, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.8K
Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.7K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

7.0K

Area of Science:

  • Biophysics
  • Cell Biology
  • Biomedical Engineering

Background:

  • High-throughput cell mechanical characterization approaches conventional flow cytometry.
  • Label-free cell analysis offers sensitivity but lacks molecular specificity.

Purpose of the Study:

  • To develop an integrated instrument combining fluorescence imaging and deformability cytometry.
  • To enhance specificity in high-throughput cell mechanical characterization.

Main Methods:

  • Development of a real-time 1D-imaging fluorescence and deformability cytometry (RT-FDC) instrument.
  • Utilizing subcellular fluorescence localization for cell identification.
  • Applying RT-FDC in an RNA interference screen to assess mechanical changes.

Main Results:

  • Demonstrated the combined capabilities of fluorescence and deformability cytometry in a single platform.
  • Successfully identified mitotic cells using subcellular fluorescence.
  • Detected mechanical changes in cells during an RNA interference screen.

Conclusions:

  • The RT-FDC instrument integrates cell mechanics and molecular identification for enhanced specificity.
  • This novel approach opens new research avenues in cell biology and biophysics.
  • RT-FDC is a powerful tool for high-throughput screening and cell analysis.