Related Experiment Video

Updated: Feb 12, 2026

An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

35.9K

SGK1 inhibition-induced autophagy impairs prostate cancer metastasis by reversing EMT

Weiwei Liu1, Xuchu Wang1, Yiyun Wang1

  • 1Department of Laboratory Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Journal of Experimental & Clinical Cancer Research : CR
|April 4, 2018
PubMed
Abstract

Insights

Serum and glucocorticoid-regulated kinase 1 (SGK1) promotes prostate cancer metastasis by driving epithelial-mesenchymal transition (EMT). Inhibiting SGK1, especially with dual targeting of mTOR, reduces metastasis through autophagy, offering new therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Metastasis

Background:

  • SGK1 is recognized as a tumor-promoting gene.
  • Its specific role in cancer metastasis regulation remains largely unexplored.

Purpose of the Study:

  • To investigate the function and mechanisms of SGK1 in prostate cancer (PCa) metastasis.
  • To explore SGK1's role in regulating epithelial-mesenchymal transition (EMT) and autophagy.

Main Methods:

  • Utilized human PCa cell lines and PC3 xenografts.
  • Employed wound healing, migration, and invasion assays.
  • Performed western blotting, immunofluorescence, and immunohistochemistry.

Main Results:

  • SGK1 expression correlates with PCa progression and metastasis.
  • SGK1 inhibition attenuated EMT and metastasis; SGK1 overexpression promoted invasion and migration.
  • SGK1 inhibition induced anti-metastatic effects via autophagy-mediated EMT repression through Snail downregulation.
  • Dual inhibition of mTOR and SGK1 synergistically enhanced anti-metastatic effects.

Conclusions:

  • SGK1 acts as a novel metastasis-promoting gene in cancer.
  • Co-targeting SGK1 and autophagy presents a promising strategy to restrain cancer progression by amplifying anti-metastatic effects.
Keywords:
AutophagyEMTMetastasisProstate cancerSGK1

More Related Videos

Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model06:32

Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

Published on: November 4, 2022

6.9K
Establishment and Analysis of Three-Dimensional 3D Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts07:21

Establishment and Analysis of Three-Dimensional 3D Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts

Published on: February 3, 2020

9.9K

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.9K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.3K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.6K
Mechanisms of Retrovirus-induced Cancers01:51

Mechanisms of Retrovirus-induced Cancers

Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
7.0K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.8K
Reversible and Irreversible Processes01:14

Reversible and Irreversible Processes

The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
5.9K