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Isolation of Soil Microorganisms Using iChip Technology
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Hymenobacter terrigena sp. nov., isolated from soil
Jeong-Eun Ohn1, Leonid N Ten2, Kyeung Il Park3
1School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea.
Journal of Microbiology (Seoul, Korea)
|April 4, 2018
Summary
A novel aerobic bacterium, Hymenobacter terrigena sp. nov. S1-2-2-5T, was identified from Korea. This Gram-negative rod represents a new species within the Hymenobacter genus, distinct from known relatives.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- The genus Hymenobacter, belonging to the Cytophagaceae family within the phylum Bacteroidetes, comprises various Gram-negative bacteria.
- Accurate taxonomic classification of bacterial isolates is crucial for understanding microbial diversity and ecological roles.
Purpose of the Study:
- To taxonomically characterize a novel bacterial strain, S1-2-2-5T, isolated from the Republic of Korea.
- To determine the phylogenetic and genotypic relationship of strain S1-2-2-5T to existing Hymenobacter species.
Main Methods:
- Polyphasic taxonomic characterization including 16S rRNA gene sequence analysis.
- Determination of DNA-G+C content, respiratory quinone analysis, fatty acid profiling, and polar lipid analysis.
- DNA-DNA hybridization assays to assess genetic relatedness with closely related species.
Main Results:
- Strain S1-2-2-5T showed highest 16S rRNA gene sequence similarity to Hymenobacter terrae DG7AT (98.2%).
- Chemotaxonomic data (menaquinone MK-7, major fatty acids, polar lipids) supported its placement within the genus Hymenobacter.
- Low DNA-DNA relatedness values (≤49.5%) with related Hymenobacter species confirmed its status as a distinct species.
Conclusions:
- Strain S1-2-2-5T is phenotypically, genotypically, and chemotaxonomically distinct from its closest neighbors.
- The proposed name Hymenobacter terrigena sp. nov. is introduced for this novel species.
- The type strain is S1-2-2-5T (= KCTC 52737T = JCM 32195T).
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