Determination of leaf respiration in the light: comparison between an isotopic disequilibrium method and the Laisk...

Quantification of leaf respiration is important for understanding plant physiology and ecosystem biogeochemical processes. Leaf respiration continues in the light (R_L ) but supposedly at a lower rate than in the dark (R_Dk ). However, there is no method for direct measurement of R_L and the available methods require nonphysiological measurement conditions. A method based on isotopic disequilibrium quantified R_L (R_L13C ) and mesophyll conductance of young and old fully expanded leaves of six species. R_L13C was compared to R_L determined by the Laisk method (R_L Laisk ) on the very same leaves with a minimum time lag. R_L 13C and R_L Laisk were generally lower than R_Dk , and were not significantly affected by leaf ageing. R_L Laisk and R_L 13C were positively correlated (r²  = 0.35), and both were positively correlated with R_Dk (r²  ≥ 0.6). R_L Laisk was systematically lower than R_L 13C by 0.4 μmol m^-2  s^-1 . Using A/C_c instead of A/C_i curves, a higher photocompensation point Γ* (by 5 μmol mol^-1 ) was found but no influence on R_L Laisk estimates was observed. The results imply that the Laisk method underestimates actual R_L significantly, probably related to the measurement condition of low CO₂ and irradiance. The isotopic disequilibrium method is useful for assessing responses of R_L to irradiance and CO₂ , improving our mechanistic understanding of R_L .