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Updated: Feb 12, 2026

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
Published on: January 15, 2011
Thermostable xylanase inhibits and disassembles Pseudomonas aeruginosa biofilms
Jin-Hyung Lee1, Yong-Guy Kim1, Jintae Lee1
1a School of Chemical Engineering , Yeungnam University , Gyeongsan , Republic of Korea.
Abstract:
Pseudomonas aeruginosa biofilms are problematic and play a critical role in the persistence of chronic infections because of their ability to tolerate antimicrobial agents. In this study, various cell-wall degrading enzymes were investigated for their ability to inhibit biofilm formation of two P. aeruginosa strains, PAO1 and PA14. Xylanase markedly inhibited and detached P. aeruginosa biofilms without affecting planktonic growth. Xylanase treatment broke down extracellular polymeric substances and decreased the viscosity of P. aeruginosa strains. However, xylanase treatment did not change the production of pyochelin, pyocyanin, pyoverdine, the Pseudomonas quinolone signal, or rhamnolipid. In addition, the anti-biofilm activity of xylanase was thermally stable for > 100 days at 45°C. Also, xylanase showed anti-biofilm activity against one methicillin-resistance Staphylococcus aureus and two Escherichia coli strains.
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