Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An iC3b receptor on Candida albicans: structure, function, and correlates for pathogenicity.

B J Gilmore1, E M Retsinas, J S Lorenz

  • 1Department of Pediatrics, University of Minnesota, Minneapolis.

The Journal of Infectious Diseases
|January 1, 1988
PubMed
Summary

The yeast Candida albicans possesses surface receptors for the complement fragment iC3b, which inhibit phagocytosis and promote pathogenicity. Blocking these receptors enhances phagocytosis, offering a potential therapeutic target.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recombinant PhpA protein, a unique histidine motif-containing protein from Streptococcus pneumoniae, protects mice against intranasal pneumococcal challenge.

Infection and immunity·2001
Same author

Fungal morphogenesis and virulence.

Medical mycology·2001
Same author

RGD-mediated adhesion in fungal pathogens of humans, plants and insects.

Current opinion in microbiology·2000
Same author

C3 as substrate for adhesion of Streptococcus pneumoniae.

The Journal of infectious diseases·2000
Same author

Novel purification scheme and functions for a C3-binding protein from Streptococcus pneumoniae.

Biochemistry·2000
Same author

A pneumococcal protein that elicits interleukin-8 from pulmonary epithelial cells.

The Journal of infectious diseases·2000

Area of Science:

  • Immunology
  • Microbiology
  • Mycology

Background:

  • Membrane receptors for complement fragments are crucial for host defense in mammals.
  • These receptors typically trigger phagocytosis to clear microbial invaders.

Purpose of the Study:

  • To investigate the presence and function of complement receptors on the yeast Candida albicans.
  • To determine the role of these receptors in C. albicans pathogenicity and host immune response.

Main Methods:

  • Binding assays using monoclonal antibodies to identify and characterize yeast surface receptors.
  • Experiments to assess the effect of receptor blockade on phagocytosis by human leukocytes.
  • Analysis of receptor expression under different growth conditions (mycelial transformation, glucose concentration).

Related Experiment Videos

Main Results:

  • Candida albicans expresses surface receptors for the complement fragment iC3b.
  • These receptors share homology with the alpha-chain of human neutrophil receptors.
  • Receptor expression is induced by mycelial transformation and high glucose.
  • Blockade of these receptors significantly increases phagocytosis of C. albicans.
  • The yeast receptors impair phagocytic uptake by human polymorphonuclear leukocytes by binding iC3b.

Conclusions:

  • Candida albicans utilizes complement receptors to evade phagocytosis, contributing to its pathogenicity.
  • These receptors represent a novel target for therapeutic strategies against C. albicans infections.