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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Deformylation reaction-based probe for in vivo imaging of HOCl

Peng Wei1, Wei Yuan1, Fengfeng Xue1

  • 1Department of Chemistry , Collaborative Innovation Center of Chemistry for Energy Materials , Fudan University , 220 Handan Road , Shanghai 200433 , China .

Chemical Science
|April 6, 2018
PubMed

Insights

A new near-infrared probe, FDOCl-1, enables sensitive detection of hypochlorous acid (HOCl) in vivo. This breakthrough addresses challenges in diagnosing diseases like arthritis and atherosclerosis by visualizing HOCl levels.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Medical Diagnostics

Background:

  • Hypochlorous acid (HOCl) is crucial for immune response but linked to diseases like atherosclerosis and rheumatoid arthritis.
  • Accurate in vivo detection of HOCl is challenging due to the absence of effective probes.
  • Developing sensitive and selective HOCl detection methods is vital for disease diagnosis and monitoring.

Purpose of the Study:

  • To develop a novel near-infrared (NIR) emissive probe for sensitive and selective in vivo detection of hypochlorous acid (HOCl).
  • To investigate a new deformylation mechanism for HOCl detection.
  • To demonstrate the probe's utility in a relevant disease model.

Main Methods:

  • Synthesis of a methylene blue derivative-based NIR "turn-on" probe, FDOCl-1.
  • Characterization of FDOCl-1's selectivity and sensitivity towards HOCl using spectroscopic methods.
  • In vitro and in vivo experiments, including application in a mouse arthritis model.

Main Results:

  • FDOCl-1 exhibits rapid and highly selective "turn-on" detection of HOCl via a novel deformylation mechanism.
  • The probe displays significant changes in color and NIR emission upon reaction with HOCl.
  • Successful in vivo imaging of HOCl in a mouse arthritis model was achieved.

Conclusions:

  • FDOCl-1 is a promising tool for the sensitive and selective detection of HOCl in biological systems.
  • The developed probe facilitates in vitro and in vivo visualization of HOCl, aiding in the study of HOCl-associated diseases.
  • This work offers a new strategy for developing advanced probes for disease diagnostics.

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