Polaromonas ginsengisoli sp. nov., isolated from ginseng field soil

A Gram-reaction-negative, strictly aerobic, milky-white and rod-shaped bacterium (designated Gsoil 115^T) isolated from ginseng field soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 115^T grew optimally at 30 °C and at pH 7.0 on Reasoner's 2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gsoil 115^T belongs to the genus Polaromonas and was most closely related to Polaromonaseurypsychrophila B717-2^T (98.6 %), Polaromonasvacuolata 34-P^T (98.3 %), Polaromonasjejuensis NBRC 106434^T (98.1 %), Polaromonas aquatic CCUG 39402^T (97.7 %) and Polaromonascryoconiti Cr4-35^T (97.5 %). The DNA G+C content was 60.9 mol%. The DNA-DNA hybridization relatedness between strain Gsoil 115^T and P. eurypsychrophila B717-2^T, P. vacuolata 34-P^T, P. jejuensis NBRC 106434^T, P. aquatic CCUG 39402^T and P. cryoconiti Cr4-35^T were 31.2, 21.6, 16.9, 8.7 and 10.1 %, respectively. The major polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The sole respiratory quinone was Q-8. The major fatty acids were C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), which supported the affiliation of strain Gsoil 115^T to the genus Polaromonas. Moreover, the physiological, biochemical and low level of DNA-DNA relatedness value allowed the phenotypic and genotypic differentiation of strain Gsoil 115^T from the recognized species of the genus Polaromonas. Therefore, strain Gsoil 115^T represents a novel species of the genus Polaromonas, for which the name Polaromonas ginsengisoli sp. nov. is proposed, with the type strain Gsoil 115^T (LMG 23393^T=KCTC 12577^T).