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Related Experiment Videos

Histamine receptors on rat ocular surface.

M Umemoto1, H Tanaka, H Miichi

  • 1Research and Development Division, Rohto Pharmaceutical Co., Ltd., Osaka, Japan.

Ophthalmic Research
|January 1, 1987
PubMed
Summary

Histamine increases microvascular permeability in rat eyes primarily through H1-receptors. Blocking these receptors with chlorpheniramine reduced this effect, while H2-receptor blockers had no impact.

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Area of Science:

  • Ophthalmology
  • Pharmacology
  • Histamine Receptor Research

Background:

  • Histamine plays a role in ocular surface responses.
  • The specific histamine receptors (H1 and H2) involved in ocular microvascular permeability are not fully elucidated.

Purpose of the Study:

  • To investigate the roles of histamine H1- and H2-receptors in mediating histamine-induced microvascular permeability on the rat ocular surface.

Main Methods:

  • Administered histamine to rat ocular surfaces to measure microvascular permeability.
  • Pretreated rats with chlorpheniramine (H1 antagonist) and cimetidine (H2 antagonist) to assess their effects.
  • Utilized dimaprit, a selective H2-receptor agonist, to evaluate H2-receptor function.
  • Examined the impact of antagonists on immediate hypersensitivity-induced permeability.

Main Results:

  • Histamine induced a dose-dependent increase in ocular microvascular permeability.
  • Chlorpheniramine significantly inhibited histamine's effect, while cimetidine did not.
  • No additive inhibitory effect was observed when both antagonists were combined.
  • Dimaprit failed to induce vasopermeability.
  • Chlorpheniramine reduced immediate hypersensitivity-related permeability, but cimetidine did not.

Conclusions:

  • The microvascular permeability response to histamine on the rat ocular surface is predominantly mediated by H1-receptors.
  • H2-receptors do not appear to play a significant role in this specific response.

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