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Sequence Analysis of IncA/C and IncI1 Plasmids Isolated from Multidrug-Resistant Salmonella Newport Using

Guojie Cao1,2, Marc Allard2, Maria Hoffmann2

  • 11 Department of Nutrition and Food Science, Joint Institute for Food Safety and Applied Nutrition, University of Maryland , College Park, Maryland.

Foodborne Pathogens and Disease
|April 6, 2018
PubMed

Insights

Multidrug-resistant Salmonella Newport strains carry diverse IncA/C plasmids with various antimicrobial resistance genes. These plasmids contribute to the spread of resistance, with similar IncA/C plasmids clustering together phylogenetically.

Area of Science:

  • Microbiology
  • Genomics
  • Molecular Biology

Background:

  • Multidrug-resistant (MDR) plasmids are key drivers of antimicrobial resistance gene dissemination.
  • IncA/C plasmids are significant in carrying resistance genes in bacterial pathogens.

Purpose of the Study:

  • To characterize antimicrobial resistance gene compositions in IncA/C plasmids from animal-derived MDR Salmonella Newport.
  • To investigate the spread mechanisms of IncA/C plasmids within Salmonella Newport.

Main Methods:

  • Complete nucleotide sequencing of six MDR Salmonella Newport strains using PacBio RSII.
  • Comparative analysis of IncA/C plasmid sequences, including reference plasmids and draft chromosomes.
  • Prokaryotic Genome Annotation Pipeline (PGAP) for open reading frame assignment.

Main Results:

  • All studied IncA/C plasmids, except one, carried various resistance genes including floR, tetA, tetR, strA, strB, sul, mer, blaCMY-2, and sugE.
  • MDR Salmonella Newport strains with similar IncA/C plasmids showed clustering in phylogenetic analysis based on chromosome sequences.
  • One IncA/C plasmid demonstrated conjugative transfer capability.

Conclusions:

  • Animal-derived Salmonella Newport IncA/C plasmids harbor diverse resistance genes, contributing to MDR.
  • Genomic analysis reveals phylogenetic relationships and suggests potential spread mechanisms for IncA/C plasmids in Salmonella Newport Lineage II.
Keywords:
IncA/CIncI1Salmonella Newportmultidrug resistance

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