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Binding efficacy of tRNA with folic acid-PAMAM nanoparticles
P Chanphai1, H A Tajmir-Riahi1
1Department of Chemistry-Biochemistry and Physics, University of Québec at Trois-Rivières, C. P. 500, Trois-Rivières, Québec G9A 5H7, Canada.
Abstract:
tRNA binding efficacy to folic acid-PAMAM nanoparticles was determined, using multiple spectroscopic methods, thermodynamic analysis and transmission electron microscopy (TEM). The structural analysis showed tRNA binds folic acid-PAMAM through H-bonding, hydrophobic and van der Waals contacts. As PAMAM size increases the binding efficacy and the stability of tRNA conjugates are enhanced. TEM analysis showed major tRNA morphological changes, upon folic acid-PAMAM complexation. Folic acid-PAMAM nanoparticles can transport tRNA in vitro.
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