Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and formation of microplasmin.

H L Wu1, G Y Shi, R C Wohl

  • 1Biochemistry Department, Medical College, National Cheng-Kung University, Taiwan, Republic of China.

Proceedings of the National Academy of Sciences of the United States of America
|December 1, 1987
PubMed
Summary

Researchers elucidated the structure of human microplasmin, revealing it comprises two polypeptide chains. This study details its composition, molecular weight, and charge properties, offering insights into its formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil <i>S100a9</i> Deficiency Protects against Periodontal Bone Loss.

Journal of dental research·2026
Same author

[Application of CA<sub>125</sub> elimination rate constant K score in prognostic forecast of patients undergoing interval debulking surgery for high grade serous ovarian cancer].

Zhonghua fu chan ke za zhi·2025
Same author

[Short-term outcomes of all-inside endoscopic running locked stitch technique for acute achilles tendon ruptures].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

[Research progress on the role of glucose in regulating immune homeostasis].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023
Same author

[Effect and mechanism of glucocorticoids in preventing stenosis after esophageal endoscopic submucosal dissection].

Zhonghua yi xue za zhi·2022
Same author

[Role of lymphocytes in the pathogenesis of autoimmune hepatitis].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Chemistry

Background:

  • Plasmin is a key enzyme in fibrinolysis.
  • Understanding plasmin derivatives like microplasmin is crucial for studying clot lysis.
  • Previous structural data on microplasmin was limited.

Purpose of the Study:

  • To determine the detailed structure of human microplasmin.
  • To characterize the polypeptide composition and molecular properties of microplasmin.
  • To propose a mechanism for microplasmin formation.

Main Methods:

  • Preparation of human microplasmin from plasmin in alkaline solution.
  • Analysis of polypeptide chain composition.
  • Calculation of molecular weight from primary sequence.

Related Experiment Videos

  • Assessment of charge and hydrophobicity.

    • Main Results:

    • Microplasmin consists of two polypeptide chains linked by disulfide bonds.
    • One chain is the plasmin B chain (230 amino acids); the other is the COOH-terminal part of the plasmin A chain (31 amino acids).
    • Microplasmin has a molecular weight of 28,635 Da, is more positively charged, and more hydrophobic than plasminogen.

    Conclusions:

    • The structure of human microplasmin has been elucidated.
    • Microplasmin formation likely involves autolysis and disulfide bond scrambling in alkaline conditions.
    • These findings contribute to the understanding of plasmin structure-function relationships.