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Unique GH18 chitinase from Euglena gracilis: full-length cDNA cloning and characterization of its catalytic domain

Toki Taira1, Chika Gushiken1, Kobeni Sugata1

  • 1a Department of Bioscience and Biotechnology , University of the Ryukyus , Okinawa , Japan.

Bioscience, Biotechnology, and Biochemistry
|April 7, 2018
PubMed

Keywords:
CBM: carbohydrate binding module familyCNF: chitin nanofiberEST: expressed sequence tagEgChi: Euglena gracilis chitinaseEuglena gracilisExcavataGH: glycoside hydrolase familyGlcNAc: N-acetylglucosamineGlxChiB: gazyumaru latex chitinase-BHPLC: high performance liquid chromatographyKH: Koren-HutnerPCR: polymerase chain reactionPDA: potato dextrose broth with agarPLChiA: pineapple leaf chitinase-ARACE: rapid amplification of cDNA endsSDS-PAGE: sodium dodecyl sulfate-polyacrylamide gel electrophoresisfamily 18 chitinase

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