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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Metabolic profiling of four South African herbal teas using high resolution liquid chromatography-mass spectrometry
Florence Malongane1, Lyndy J McGaw2, Hlengilizwe Nyoni3
1Department Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Private Bag X6, Florida 1710, South Africa.
Abstract:
Increased preference to herbal drinks has led to global interest in the use and production of different plant species for the preparation of various drink formulations. Medicinal properties derived from bioactive compounds remain the main driver of choice for herbal teas. This study determined the chemical variation in honeybush, rooibos, special and bush tea, profiled compounds responsible for such differences and compared their peak areas. Nuclear magnetic resonance and high resolution liquid chromatography-mass spectrometry were used to determine compound variation and profiling. Principal component analysis and partial-least square multivariate statistical analysis showed distinct differences (P < 0.05) between the different types of herbal teas. Detected compounds included flavonoids, phenolics, lignans, megastigmane glycoside, most of which possess health benefits. The findings showed that South African herbal teas could play a vital role as health promoting drinks, and that bush tea and special tea are phytochemically comparable with other commercialized herbal teas.
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