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Related Experiment Videos

Molecular crowding on the cell surface.

T A Ryan1, J Myers, D Holowka

  • 1Department of Physics, Cornell University, Ithaca, NY 14853.

Science (New York, N.Y.)
|January 1, 1988
PubMed
Summary
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Steric interactions between proteins on cell surfaces are significant, impacting their spatial distribution. Accounting for these interactions reveals unexpected thermodynamic activity even at low concentrations.

Area of Science:

  • Cell biology
  • Biophysics
  • Statistical thermodynamics

Background:

  • Cell surface proteins play crucial roles in cellular functions.
  • Understanding protein interactions is key to deciphering cellular processes.
  • Crowded environments on cell surfaces can significantly influence protein behavior.

Purpose of the Study:

  • To investigate the impact of steric interactions on protein distribution on living cell surfaces.
  • To develop a statistical thermodynamic model that accurately represents protein spatial organization.
  • To explore the thermodynamic activity of cell surface proteins under crowded conditions.

Main Methods:

  • Measurements of equilibrium spatial distributions of proteins.
  • Application of potential gradients to study protein movement.

Related Experiment Videos

  • Statistical thermodynamic analysis incorporating steric exclusion.

    • Main Results:

    • Strong steric interactions among proteins on crowded cell surfaces were identified.
    • A model including steric exclusion accurately predicts the fraction of accessible surface occupied by mobile proteins.
    • Enhanced, concentration-dependent activity coefficients were observed, indicating significant thermodynamic activity.

    Conclusions:

    • Steric exclusion is a critical factor in determining protein organization on cell surfaces.
    • The thermodynamic activity of cell surface proteins is higher than previously assumed, even at typical receptor concentrations.
    • This study provides new insights into the biophysics of crowded cellular environments.