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Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
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A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study
Weili Gong1, Lin Dai1, Huaiqiang Zhang1
1The State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Frontiers in Microbiology
|April 7, 2018
Summary
Aspergillus niger efficiently degrades xylan using a sequential enzyme system. Adding xylooligosaccharides (XOS) to cultures enhances enzyme production and shortens fermentation for biofuels.
Area of Science:
- Biotechnology
- Microbiology
- Biochemistry
Background:
- Xylan, a major hemicellulose, is abundant in biomass and can be degraded into fermentable sugars by Aspergillus niger.
- Efficient degradation of xylan is crucial for producing renewable biofuels and biochemicals.
Purpose of the Study:
- To elucidate the mechanisms of highly efficient xylan degradation, assimilation, and metabolism by Aspergillus niger.
- To analyze the roles of secreted proteins, sugar transporters, and intracellular proteins during xylan degradation.
Main Methods:
- Functional proteomics was employed to analyze proteins in Aspergillus niger An76 grown on xylan-based substrates.
- Investigated the sequential secretion of xylanolytic enzymes and the induction of transcription factors.
Main Results:
- A complete xylanolytic enzyme system, including diverse isozymes, was secreted sequentially.
- Xylooligosaccharides (XOS) were more effective than xylose in inducing the transcription activator XlnR, leading to increased xylanase activity and reduced production time.
- Substituted XOS enhanced the abundance of side-chain-degrading enzymes and key metabolic pathway components.
Conclusions:
- The findings suggest that adding XOS to filamentous fungi cultures can optimize xylan-degrading enzyme profiles and reduce fermentation times for industrial applications.
- This strategy holds potential for improving the efficiency of biofuel and biochemical production from biomass.
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