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Related Experiment Video

Updated: Feb 12, 2026

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Published on: March 15, 2012

A Highly Efficient Xylan-Utilization System in Aspergillus niger An76: A Functional-Proteomics Study.

Weili Gong¹, Lin Dai¹, Huaiqiang Zhang¹

¹The State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.

Frontiers in Microbiology

|April 7, 2018

Summary

Aspergillus niger efficiently degrades xylan using a sequential enzyme system. Adding xylooligosaccharides (XOS) to cultures enhances enzyme production and shortens fermentation for biofuels.

Keywords:

Aspergillus niger An76 sugar transporter transcription activator XlnR xylan xylan-degrading isoenzyme

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Last Updated: Feb 12, 2026

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Area of Science:

Biotechnology
Microbiology
Biochemistry

Background:

Xylan, a major hemicellulose, is abundant in biomass and can be degraded into fermentable sugars by Aspergillus niger.
Efficient degradation of xylan is crucial for producing renewable biofuels and biochemicals.

Purpose of the Study:

To elucidate the mechanisms of highly efficient xylan degradation, assimilation, and metabolism by Aspergillus niger.
To analyze the roles of secreted proteins, sugar transporters, and intracellular proteins during xylan degradation.

Main Methods:

Functional proteomics was employed to analyze proteins in Aspergillus niger An76 grown on xylan-based substrates.
Investigated the sequential secretion of xylanolytic enzymes and the induction of transcription factors.

Main Results:

A complete xylanolytic enzyme system, including diverse isozymes, was secreted sequentially.
Xylooligosaccharides (XOS) were more effective than xylose in inducing the transcription activator XlnR, leading to increased xylanase activity and reduced production time.
Substituted XOS enhanced the abundance of side-chain-degrading enzymes and key metabolic pathway components.

Conclusions:

The findings suggest that adding XOS to filamentous fungi cultures can optimize xylan-degrading enzyme profiles and reduce fermentation times for industrial applications.
This strategy holds potential for improving the efficiency of biofuel and biochemical production from biomass.