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Updated: Feb 12, 2026

Quantifying Agonist Activity at G Protein-coupled Receptors
Published on: December 26, 2011
Metals Differentially Activate Ovarian Cancer G Protein-Coupled Receptor 1 in Various Species
Yuta Mochimaru1, Jun Negishi1, Syo Murakami1
11 Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
Abstract:
Human, mouse, and zebrafish ovarian cancer G protein-coupled receptors (OGR1s) are activated by both metals and extracellular protons. In the present study, we examined whether pig, rat, chicken, and Xenopus OGR1 homologs could sense and be activated by protons and metals. We found that all homologs stimulated serum response element (SRE)-driven promoter activities when they are stimulated by protons. On the other hand, metals differentially activated the homologs. The results using chimeric receptors of human and zebrafish OGR1s indicate that the specificity of the metal-induced activation lies in the extracellular region. These results suggest that protons are an evolutionally conserved agonist of OGR1. However, the types of metals that activated the receptor differed among the homologs.
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