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Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro
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How Does Plasma Activated Media Treatment Differ From Direct Cold Plasma Treatment?
Pankaj Attri1, Ji Hoon Park1, Anser Ali1
1Department of Electrical and Biological Physics, Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, Korea.
Anti-Cancer Agents in Medicinal Chemistry
|April 7, 2018
Summary
Cold atmospheric plasma-activated media (PAM) selectively kills cancer cells with minimal damage to normal cells. Argon-Nitrogen (Ar-N2) plasma is optimal for PAM preparation, while Argon-Oxygen (Ar-O2) plasma excels in direct cancer cell treatment.
Area of Science:
- Plasma medicine
- Cancer therapy
- Cell biology
Background:
- Cold atmospheric plasma-activated media (PAM) shows promise in plasma medicine for cancer treatment.
- PAM exhibits selective anti-cancer capacity both in vitro and in vivo.
Purpose of the Study:
- Investigate optimal conditions for generating PAM for selective cancer cell deactivation.
- Minimize damage to normal cells during plasma-activated media treatment.
Main Methods:
- Analyzed radicals in air via optical emission spectroscopy.
- Assessed radical concentration in culture media through chemical analysis.
- Determined PAM toxicity using MTT assay.
Main Results:
- Argon (Ar) plasma showed the highest cancer cell death, followed by Ar-N2, then Ar-O2 plasma.
- Ar-N2 plasma yielded higher reactive nitrogen species (RNS) in gas and media.
- Reactive oxygen species (ROS) were identified as the primary contributors to cancer cell death.
Conclusions:
- Argon-Oxygen (Ar-O2) plasma is most effective for direct cancer cell treatment.
- Argon-Nitrogen (Ar-N2) plasma is optimal for PAM preparation, selectively killing cancer cells with minimal normal cell impact at low treatment times (1-2 min).
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