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Updated: Feb 12, 2026
07:08
Modeling and Imaging 3-Dimensional Collective Cell Invasion
Published on: December 7, 2011
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Probing three-dimensional collective cancer invasion with DIGME
1Department of Physics, Oregon State University, Weniger Hall, Corvallis, OR USA.
Cancer Convergence
|April 7, 2018
Summary
Researchers developed a new method to engineer 3D tissue organoids and study cancer invasion. The study reveals that extracellular matrix geometry significantly controls collective cancer cell movement in 3D environments.
Area of Science:
- Biophysics
- Developmental Biology
- Cancer Research
Background:
- Multicellular pattern formation is crucial in development, cancer, and healing.
- The influence of extracellular matrix (ECM) geometry on 3D cancer invasion remains unclear.
Purpose of the Study:
- To investigate the role of ECM micro-to-meso scale geometry in 3D collective cancer invasion.
- To develop a novel tool for engineering 3D tissue organoids within patterned microenvironments.
Main Methods:
- Developed Diskoid In Geometrically Micropatterned ECM (DIGME) strategy.
- Engineered 3D tissue organoid shape, ECM heterogeneity, and collagen fiber alignment simultaneously.
- Studied 3D invasion of MDA-MB-231 cancer cell diskoids in engineered collagen matrices.
Main Results:
- Collective cancer invasion is significantly regulated by ECM micro-to-meso scale geometry.
- DIGME enables simultaneous control over organoid shape, ECM heterogeneity, and fiber alignment.
- Demonstrated the utility of DIGME in studying cancer cell invasion dynamics.
Conclusions:
- DIGME is a powerful and simple tool for probing 3D tissue organoid dynamics.
- Physically patterned microenvironments, controlled by DIGME, influence collective cancer invasion.
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