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Atriopeptins decrease resting and hormone-elevated cytosolic Ca in cultured mesangial cells.

A Hassid1

  • 1Department of Pharmacology, University of Tennessee, Memphis 38163.

The American Journal of Physiology
|December 1, 1987
PubMed
Summary
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Atrial peptides, like atriopeptin-23, lower calcium levels in mesangial cells. These peptides also modulate hormone-induced calcium changes, impacting cellular signaling pathways.

Area of Science:

  • Nephrology
  • Cell Biology
  • Cardiovascular Physiology

Background:

  • Mesangial cells play a crucial role in kidney function.
  • Cytosolic calcium (Ca) levels are critical regulators of cellular processes.
  • Atrial peptides are known for their roles in cardiovascular homeostasis.

Purpose of the Study:

  • To investigate the impact of atrial peptides on intracellular calcium dynamics in cultured mesangial cells.
  • To determine the dose-dependent effects of atriopeptins on resting and stimulated Ca levels.
  • To elucidate the modulatory role of atrial peptides on hormone-induced Ca transients.

Main Methods:

  • Utilized fura-2 for precise measurement of cytosolic Ca levels.
  • Employed cultured, adherent mesangial cells for experimental models.

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  • Administered varying concentrations of atriopeptin-23 and atriopeptin-21.
  • Stimulated cells with vasopressin and angiotensin II to induce Ca transients.

    • Main Results:

    • Atriopeptin-23 dose-dependently decreased resting Ca levels by up to 30% (EC50 ~30 pM).
    • Atriopeptin-21 showed similar reduction but was ~30-fold less potent.
    • 100 nM atriopeptin-23 inhibited vasopressin- and angiotensin II-induced Ca transients, but not at lower concentrations.
    • Atriopeptin-21 did not affect vasopressin-induced Ca transients.

    Conclusions:

    • Atrial peptides act as significant modulators of resting Ca levels in mesangial cells.
    • Atriopeptins can modulate hormone-stimulated Ca increases under specific conditions.
    • These findings highlight a novel role for atrial peptides in regulating mesangial cell signaling.