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Related Experiment Videos

Calcium transport in turtle bladder.

S Sabatini1, N A Kurtzman

  • 1Department of Internal Medicine, School of Medicine, Texas Tech University Health Sciences Center, Lubbock 79430.

The American Journal of Physiology
|December 1, 1987
PubMed
Summary
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Turtle bladder studies reveal calcium transport mechanisms. Ouabain reverses net calcium flux, indicating active transport possibly driven by Na+-K+-ATPase, with the apical membrane acting as the primary barrier.

Area of Science:

  • Physiology
  • Membrane Transport
  • Renal Physiology

Background:

  • Calcium homeostasis is crucial for various physiological processes.
  • Understanding ion transport in epithelial tissues like the bladder is essential for comprehending overall physiological regulation.

Purpose of the Study:

  • To investigate the mechanisms of unidirectional calcium (45Ca) transport across the turtle bladder.
  • To elucidate the role of apical and basolateral membranes in calcium flux.
  • To identify potential transporters involved in calcium movement.

Main Methods:

  • Measurement of unidirectional 45Ca fluxes under open-circuit and short-circuit conditions.
  • Application of pharmacological agents like ouabain and amiloride to assess transporter activity.

Related Experiment Videos

  • Manipulation of mucosal and serosal sodium concentrations to evaluate sodium-dependent calcium transport.

    • Main Results:

    • Net calcium flux (JnetCa) was secretory in the open-circuited state, reversing to absorptive in the presence of ouabain.
    • Amiloride significantly reduced both secretory and absorptive fluxes.
    • Removal of mucosal sodium led to calcium absorption, while removal of serosal sodium resulted in calcium secretion.
    • Tissue 45Ca content was lower when the isotope was in the mucosal bath, indicating the apical membrane is a resistance barrier.

    Conclusions:

    • A model is proposed involving a basolateral Ca2+-ATPase and a mucosal sodium-calcium antiporter for calcium transport.
    • The Na+-K+-ATPase likely provides energy for calcium transport.
    • Ouabain's effect suggests increased intracellular sodium inhibits the mucosal sodium-calcium exchanger, revealing active serosal transport.