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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Antiprotozoan sesterterpenes and triterpenes isolated from two Ghanaian mushrooms
Frederick Annang1, Ignacio Pérez-Victoria1, Theresa Appiah2
1Fundación MEDINA, Avda. del Conocimiento 34, 18016 Armilla, Granada, Spain.
Abstract:
Bioassay-guided compound isolation led to the discovery of two new scalarane sesterterpenes (1 and 2) and two new triterpenes (3 and 4) from two mushroom species, Pleurotus ostreatus (edible) and Scleroderma areolatum, collected from Ghana. Their structures, including absolute stereochemistry, were established by spectroscopic methods, particularly (+)-ESI-TOF mass spectrometry and 1D and 2D NMR. The four compounds exhibited IC50 values of 1.65-7.63 μM against Plasmodium falciparum 3D7 and 5.04-13.65 μM against Trypanosoma cruzi Tulahuen C4 parasites and were also non-cytotoxic against HepG2 tumoral human liver cells. This is the first report describing the isolation of sesterterpenes belonging to the scalarane structural class from a terrestrial source.
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