Effects of elevated ozone concentration and nitrogen addition on ammonia stomatal compensation point in a poplar...

The stomatal compensation point of ammonia (χ_s) is a key factor controlling plant-atmosphere NH₃ exchange, which is dependent on the nitrogen (N) supply and varies among plant species. However, knowledge gaps remain concerning the effects of elevated atmospheric N deposition and ozone (O₃) on χ_s for forest species, resulting in large uncertainties in the parameterizations of NH₃ incorporated into atmospheric chemistry and transport models (CTMs). Here, we present leaf-scale measurements of χ_s for hybrid poplar clone '546' (Populusdeltoides cv. 55/56 x P. deltoides cv. Imperial) growing in two N treatments (N0, no N added; N50, 50 kg N ha^-1 yr^-1 urea fertilizer added) and two O₃ treatments (CF, charcoal-filtered air; E-O₃, non-filtered air plus 40 ppb) for 105 days. Our results showed that χ_s was significantly reduced by E-O₃ (41%) and elevated N (19%). The interaction of N and O₃ was significant, and N can mitigate the negative effects of O₃ on χ_s. Elevated O₃ significantly reduced the light-saturated photosynthetic rate (A_sat) and chlorophyll (Chl) content and significantly increased intercellular CO₂ concentrations (Ci), but had no significant effect on stomatal conductance (g_s). By contrast, elevated N did not significantly affect all measured photosynthetic parameters. Overall, χ_s was significantly and positively correlated with A_sat, g_s and Chl, whereas a significant and negative relationship was observed between χ_s and Ci. Our results suggest that O₃-induced changes in A_sat, Ci and Chl may affect χ_s. Our findings provide a scientific basis for optimizing parameterizations of χ_s in CTMs in response to environmental change factors (i.e., elevated N deposition and/or O₃) in the future.