Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Impact Loading01:19

Impact Loading

718
Impact loading occurs when a moving object collides with a stationary structure, such as a rod with a uniform cross-sectional area fixed at one end. Under these conditions, the rod absorbs the kinetic energy from the striking object, leading to deformation and subsequent stress development. As the rod returns to its original position and reaches maximum stress, the absorbed energy, initially manifested as kinetic energy, transforms entirely into strain energy.
In cases of elastic deformation,...
718
Distributed Loads01:19

Distributed Loads

981
Distributed loads are a common type of load that engineers and scientists encounter in various practical situations. Distributed loads often refer to a type of load spread over a surface or a structure and can be modeled as continuous force per unit area.
For example, consider a bookshelf filled with books stacked vertically adjacent to each other. The weight of the books is evenly distributed over the length of the shelf. As a result, the pressure at different locations on the surface of the...
981
Eccentric Loading01:16

Eccentric Loading

1.0K
Eccentric loading is a crucial concept in the study of structural engineering and mechanics, particularly when analyzing the stability and stress distribution in columns. Unlike centric loading, where the force is applied along the centroidal axis, causing uniform compression, eccentric loading occurs when a force is applied off-center. This off-center application introduces not only direct compressive stress but also bending stress, significantly influencing the column's behavior under...
1.0K
Load along a Single Axis01:29

Load along a Single Axis

648
In structural engineering, the analysis of beams subjected to varying loads is a critical aspect of understanding the behavior and performance of these structural elements. A common scenario involves a beam subjected to a combination of different load distributions.
Consider a beam of length L subjected to a varying load, which is a combination of parabolic and trapezoidal load distribution along the x-axis. In this case, it is essential to determine the resultant loads, their locations, and...
648
Stress: General Loading Conditions01:15

Stress: General Loading Conditions

597
To grasp the intricacy of real-world conditions where multiple loads are applied simultaneously to a structure, one might visualize a section passing through a specific point within a body, aligned parallel to the xy plane. This section is subjected to various forces, including original loads, normal forces, and shearing forces.
The shearing force, possessing potential directionality within the plane of the section, is simplified into two component forces running parallel to the x and y axes....
597
General Case of Eccentric Axial Loading01:12

General Case of Eccentric Axial Loading

535
Unsymmetrical bending occurs when the bending moment applied to a structural member does not align with its principal axis. This misalignment leads to complex stress distributions and deflection patterns that differ from symmetrical bending, which are essential for designing structures to withstand different loading conditions.
Consider a member subjected to equal and opposite forces that are applied along a line that does not coincide with the member's neutral axis. In unsymmetrical...
535

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the protein lattice: bacterial S-layer glycans - from structure to functional frontier.

Current opinion in microbiology·2026
Same author

Molecular insights into the dual-glycoprotein surface layer of the oral bacterium Tannerella serpentiformis.

Journal of molecular graphics & modelling·2026
Same author

Effect of Low-Level Laser Therapy on Periodontal Host Cells and a Seven-Species Periodontitis Model Biofilm.

International journal of molecular sciences·2025
Same author

Vitamin D<sub>3</sub> Modulates Inflammatory and Antimicrobial Responses in Oral Epithelial Cells Exposed to Periodontitis-Associated Bacteria.

International journal of molecular sciences·2025
Same author

A new age in structural S-layer biology: Experimental and in silico milestones.

The Journal of biological chemistry·2025
Same author

Synthesis, Microbiology, and Biophysical Characterization of Mutanofactins from the Human Oral Microbiome.

ACS central science·2025

Related Experiment Video

Updated: Feb 12, 2026

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
07:28

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Published on: June 1, 2021

5.5K

Carb loading takes proteins on a ride.

Christina Schäffer1, Paul Messner2

  • 1From the Department of NanoBiotechnology, NanoGlycobiology Unit, Universität für Bodenkultur Wien, A-1190 Vienna, Austria christina.schaeffer@boku.ac.at.

The Journal of Biological Chemistry
|April 8, 2018
PubMed
Summary
This summary is machine-generated.

Bacterial protein export relies on O-glycosylation, a modification essential for protein maturation. This study reveals how glycosyltransferases and translocation systems work together to deliver modified adhesins to the membrane.

Keywords:
adhesinbacteriaglycobiologymembrane transportpost-translational modification (PTM)

More Related Videos

V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting
11:33

V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting

Published on: December 30, 2013

23.3K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.7K

Related Experiment Videos

Last Updated: Feb 12, 2026

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells
07:28

Bead Loading Proteins and Nucleic Acids into Adherent Human Cells

Published on: June 1, 2021

5.5K
V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting
11:33

V3 Stain-free Workflow for a Practical, Convenient, and Reliable Total Protein Loading Control in Western Blotting

Published on: December 30, 2013

23.3K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.7K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacterial protein O-glycosylation is crucial for protein maturation.
  • The role of carbohydrate modifications in bacterial protein export is not well understood.

Purpose of the Study:

  • To elucidate the mechanism of posttranslational modification of a Streptococcus gordonii adhesin.
  • To develop a comprehensive model for the interplay of glycosyltransferases and the translocation system in bacterial protein export.

Main Methods:

  • Investigated the posttranslational modification of a specific Streptococcus gordonii adhesin.
  • Analyzed the interaction between glycosyltransferases and the protein translocation system.

Main Results:

  • Described the mechanism for O-glycosylation of a Streptococcus gordonii adhesin.
  • Provided the first comprehensive model detailing the interplay of glycosyltransferases and the translocation system for protein delivery to the membrane.

Conclusions:

  • Bacterial protein export is dependent on O-glycosylation.
  • A novel model highlights the coordinated function of glycosyltransferases and translocation systems in bacterial adhesin delivery.