Gestational exposure to tetrabutyltin blocks rat fetal Leydig cell development

Tetrabutyltin is a stable organotin and may exhibit endocrine disrupting properties. Herein, we investigated effects of tetrabutyltin on the development of rat fetal Leydig cells, which support differentiation of the male reproductive tract in late gestation. Female pregnant Sprague Dawley rats were gavaged with tetrabutyltin (0, 100, 200, and 500 mg/kg) from gestational day (GD) 12 to GD 21. Tetrabutyltin dose-dependently decreased testicular testosterone levels (0.756 ± 0.208 and 0.813 ± 0.277 ng/testis at the 200 and 500 mg/kg doses, respectively) compared to control (1.692 ± 0.218 ng/testis) at GD 21. Furthermore, tetrabutyltin induced fetal Leydig cell aggregation, decreased fetal Leydig cell size and cytoplasmic size at the ≥100 mg/kg doses, and downregulated the expression levels of Scarb1, Cyp17a1, and Insl3 at doses ≥100 mg/kg and Star expression at 200 mg/kg. Taking together, the present results indicated that prenatal exposure of male rats to tetrabutyltin affected fetal Leydig cell development.