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Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa

T Pini1, J P Rickard1, T Leahy1

  • 1Faculty of Science, School of Life and Environmental Sciences, The University of Sydney, NSW 2006, Australia.

Journal of Proteomics
|April 9, 2018
PubMed

Insights

Cryopreservation damages ram sperm proteins, altering 51 proteins, including chaperones. Understanding these changes is key to improving sperm cryopreservation outcomes and fertility.

Area of Science:

  • * Proteomics
  • * Reproductive Biology
  • * Cryobiology

Background:

  • * Cryopreservation causes significant damage to spermatozoa, impacting fertility.
  • * Understanding sub-lethal damage is crucial for improving cryopreservation protocols.
  • * Limited studies have used advanced mass spectrometry to analyze cryopreservation-induced sperm protein changes.

Purpose of the Study:

  • * To quantitatively profile proteomic changes in ram spermatozoa after exposure to egg yolk and cryopreservation.
  • * To identify specific proteins contributed by egg yolk-based freezing media.
  • * To characterize cryopreservation-induced alterations in ram sperm proteome using SWATH LC-MS.

Main Methods:

  • * Employed SWATH LC-MS, a data-independent acquisition mass spectrometry method.
  • * Analyzed proteomic changes in ram spermatozoa subjected to egg yolk exposure and cryopreservation.
  • * Compared protein profiles of fresh, frozen-thawed, and egg yolk-exposed spermatozoa.

Main Results:

  • * Egg yolk contributed 15 proteins to spermatozoa, including vitellogenins and apolipoproteins.
  • * Cryopreservation significantly altered the abundance of 51 proteins in ram spermatozoa.
  • * 27 proteins increased and 24 decreased in abundance; chaperones comprised 20% of lost proteins.

Conclusions:

  • * Cryopreservation induces substantial proteomic changes in ram spermatozoa, affecting cellular functions.
  • * Egg yolk-based media introduce specific proteins into spermatozoa.
  • * Identified protein alterations may impact the stress response and functionality of cryopreserved sperm.
Keywords:
CryopreservationOvineSWATHSperm

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