Ozone risk assessment is affected by nutrient availability: Evidence from a simulation experiment under free air...

Assessing ozone (O₃) risk to vegetation is crucial for informing policy making. Soil nitrogen (N) and phosphorus (P) availability could change stomatal conductance which is the main driver of O₃ uptake into a leaf. In addition, the availability of N and P could influence photosynthesis and growth. We thus postulated that the sensitivity of plants to O₃ may be changed by the levels of N and P in the soil. In this study, a sensitive poplar clone (Oxford) was subject to two N levels (N0, 0 kg N ha^-1; N80, 80 kg N ha^-1), three P levels (P0, 0 kg P ha^-1; P40, 40 kg P ha^-1; P80, 80 kg P ha^-1) and three levels of O₃ exposure (ambient concentration, AA; 1.5 × AA; 2.0 × AA) for a whole growing season in an O₃ free air controlled exposure (FACE) facility. Flux-based (POD_{0 to 6}) and exposure-based (W126 and AOT40) dose-response relationships were fitted and critical levels (CLs) were estimated for a 5% decrease of total annual biomass. It was found that N and P availability modified the dose-response relationships of biomass responses to O₃. Overall, the N supply decreased the O₃ CLs i.e. increased the sensitivity of poplar to O₃. Phosphorus alleviated the O₃-caused biomass loss and increased the CL. However, such mitigation effects of P were found only in low N and not in high N conditions. In each nutritional treatment, similar performance was found between flux-based and exposure-based indices. However, the flux-based approach was superior, as compared to exposure indices, to explain the biomass reduction when all nutritional treatments were pooled together. The best O₃ metric for risk assessments was POD₄, with 4.6 mmol m^-2 POD₄ as a suitable CL for Oxford poplars grown under various soil N and P conditions.