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Related Experiment Video

Updated: Feb 12, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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Published on: June 12, 2018

Target-activated streptavidin-biotin controlled binding probe.

Yung-Peng Wu¹, Chee Ying Chew¹, Tian-Neng Li²

¹Department of Chemistry , National Tsing Hua University , 101 Sec. 2, Kuang Fu Rd , Hsinchu 30013 , Taiwan , Republic of China .

Chemical Science

|April 10, 2018

Summary

Researchers developed a novel chemical probe utilizing streptavidin-biotin binding for enhanced detection. This innovative tool offers improved sensitivity and versatility for biological research and medical diagnostics.

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Area of Science:

Chemical Biology
Biomedical Engineering
Molecular Imaging

Background:

Target-activated chemical probes are crucial for monitoring biological processes and disease markers.
Existing probes often rely on fluorescence or caged-luciferin mechanisms, with limitations in background noise and signal amplification.
There is a need for versatile probes with enhanced sensitivity and customizable detection modalities.

Purpose of the Study:

To introduce a new class of chemical probes based on controlled streptavidin-biotin binding.
To develop a novel synthetic method for facile introduction of analyte recognition groups.
To demonstrate the probe's utility in imaging and studying cell-surface peroxynitrite.

Main Methods:

Development of a novel chemical probe system leveraging the streptavidin-biotin interaction for fluorescence turn-on.
Creation of a new synthetic strategy to enable diverse analyte recognition group incorporation.
Application of the probe for imaging secreted peroxynitrite (ONOO-) at the macrophage cell surface.

Main Results:

The streptavidin-biotin controlled binding probe exhibits minimal background noise in the "OFF" state.
The probe design allows for multiple signal amplification steps and flexible dye selection.
Successful imaging and study of cell-surface peroxynitrite in macrophages were achieved.
The probe's versatility was demonstrated for fluorescence, electrochemical, and luminescence detection modes.

Conclusions:

The streptavidin-biotin binding probe represents a significant advancement over conventional chemical probes.
The versatile design and facile synthesis allow for broad applicability in biological research and diagnostics.
This probe platform opens new avenues for studying cellular signaling and disease mechanisms.