Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free
Ana Sevilla1, Eliana Forero1, Matthew Zimmer1
1The New York Stem Cell Foundation Research Institute, New York, NY 10019, United States.
Abstract:
The human embryonic stem cell line NYSCFe001-A was derived from a day 6 blastocyst in feeder-free and antibiotic free conditions. The blastocyst was voluntarily donated for research as surplus after in vitro fertilization treatment following informed consent. The NYSCFe001-A line, registered as NYSCF100 on the NIH registry, presents normal karyotype, is mycoplasma free, expresses all the pluripotency markers and has the potential to differentiate into all three germ layers in vitro.
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