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Updated: Feb 12, 2026

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
Published on: December 13, 2014
High-frequency, high-intensity electromagnetic field effects on Saccharomyces cerevisiae conversion yields and growth
Emmanuel Bertrand1, Christophe Pasquier2, David Duchez3
1Université Clermont Auvergne, CS 60032, 63001 Clermont-Ferrand, France; Institut Pascal, UMR CNRS 6602 Team GePEB, Chemical Engineering, Applied Thermodynamics and Biosystems, BP 10448, F-63000 Clermont-Ferrand, France; Aix-Marseille Université, INRA, Polytech' Marseille, UMR 1163 Biodiversité et Biotechnologie Fongiques, 163 Avenue de Luminy, CP225, 13288 Marseille Cedex 09, France.
Abstract:
Studies of the effects of electromagnetic waves on Saccharomyces cerevisiae emphasize the need to develop instrumented experimental systems ensuring a characterization of the exposition level to enable unambiguous assessment of their potential effects on living organisms. A bioreactor constituted with two separate compartments has been designed. The main element (75% of total volume) supporting all measurement and control systems (temperature, pH, agitation, and aeration) is placed outside the exposure room whereas the secondary element is exposed to irradiation. Measurements of the medium dielectric properties allow the determination of the electromagnetic field at any point inside the irradiated part of the reactor and are consistent with numerical simulations. In these conditions, the growth rate of Saccharomyces cerevisiae and the ethanol yield in aerobic conditions are not significantly modified when submitted to an electromagnetic field of 900 and 2400 MHz with an average exposition of 6.11 V.m-1 and 3.44 V.m-1 respectively.
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