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Avicequinone B sensitizes anoikis in human lung cancer cells

Arisara Prateep1, Somruethai Sumkhemthong1, Wiranpat Karnsomwan2

  • 1Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.

Journal of Biomedical Science
|April 11, 2018
PubMed
Abstract

Insights

Avicequinone B, a natural compound, sensitizes human lung cancer cells to anoikis, a key process in metastasis. This compound reduces survival signals, offering potential for new cancer therapies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Cancer cells develop anoikis resistance to survive during metastasis.
  • Sensitizing cancer cells to anoikis is a potential therapeutic strategy.
  • Avicequinone B, a natural furanonaphthoquinone from mangroves, was investigated for its anoikis-sensitizing potential.

Purpose of the Study:

  • To investigate the potential of avicequinone B to sensitize human lung cancer cells to anoikis.
  • To evaluate the effect of avicequinone B on cancer cell viability and anchorage-independent growth.
  • To elucidate the molecular mechanisms underlying avicequinone B-induced anoikis.

Main Methods:

  • Human lung cancer cell lines (H460, H292, H23) were cultured in ultra-low attachment plates with avicequinone B.
  • Cell viability was assessed using XTT assay.
  • Anchorage-independent growth was evaluated by soft agar assay.
  • Western blot analysis was used to examine anoikis-regulating proteins.

Main Results:

  • Avicequinone B (4 μM) significantly induced anoikis and inhibited proliferation in detached lung cancer cells.
  • Treatment with avicequinone B led to reduced levels of anti-apoptotic proteins B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia 1 (Mcl-1).
  • Avicequinone B diminished integrin/focal adhesion kinase (FAK)/Proto-oncogene tyrosine-protein kinase (Src) signaling pathways.

Conclusions:

  • Avicequinone B effectively sensitizes human lung cancer cells to anoikis.
  • The mechanism involves down-regulation of anti-apoptotic proteins.
  • Avicequinone B also inhibits integrin-mediated survival signaling, contributing to anoikis sensitization.
Keywords:
AnoikisAvicequinone BFuranonaphthoquinoneLung cancerSurvival pathway

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