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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC
Published on: May 10, 2016
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Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc
Rhulani Makhuvele1,2, Kenn Foubert3, Sandra Apers3
1Toxicology and Ethnoveterinary Medicine, ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa.
The Journal of Pharmacy and Pharmacology
|April 11, 2018
Summary
Two compounds from Monanthotaxis caffra, crotepoxide and 5,6-diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene, show antimutagenic potential. These compounds may help reduce risks associated with aflatoxin B1 exposure in food and feed.
Area of Science:
- Phytochemistry
- Toxicology
- Natural Products Chemistry
Background:
- Monanthotaxis caffra leaf extract exhibits significant antimutagenic properties against aflatoxin B1.
- Aflatoxin B1 is a potent carcinogen found in contaminated food and feed, posing health risks.
Purpose of the Study:
- To isolate and identify the active antimutagenic constituents from Monanthotaxis caffra.
- To evaluate the antimutagenic potential of isolated compounds against aflatoxin B1.
Main Methods:
- Bioassay-guided fractionation using chromatographic and spectroscopic techniques.
- Ames, Vitotox, and Comet assays were employed to assess antimutagenicity.
- Investigation of the mechanism of action, including effects on biotransformation proteins.
Main Results:
- Two compounds, (+)-crotepoxide and 5,6-diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene, were isolated and identified.
- (+)-Crotepoxide demonstrated strong antimutagenicity in the Vitotox assay (IC50 = 131 μg/ml).
- 5,6-Diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene showed potent activity in the Ames assay (IC50 = 348.9 μg/plate) and inhibited aflatoxin B1 biotransformation.
Conclusions:
- Crotepoxide and 5,6-diacetoxy-1-benzoyloxymethyl-1,3-cyclohexadiene possess significant antimutagenic properties.
- These compounds offer potential as natural agents to mitigate risks from aflatoxin B1 contamination.
- Further research into their application in food and feed safety is warranted.
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