Early changes in quiescent B cell physiology subsequent to cognate and bystander interaction with helper T cells

We have assessed early changes in quiescent B cells following cognate and bystander interaction with cloned helper T cells. Variables monitored include Ia expression, blastogenesis, G0 to G1 transition, and progression through cycle. We have also assessed the antigen specificity, Ia restriction, and dependence on membrane immunoglobulin crosslinking of both generation and delivery of effectors that mediate B cell responses. The results demonstrate that antigen presentation by quiescent B cells to T cells resulting in the generation of effectors that activate B cells is Ia-restricted and dependent on antigen and an (mIgM and mIgD) crosslinking signal. However, once generated, T cell effector functions act independently of Ia haplotype to promote Ia expression, blastogenesis, and G0 to G1 transition by most small B cells. Although these responses can be mediated by T cell supernatants, further progression of B cells through S, G2 and M is only efficient when Th cells are present in cultures. Thus, results suggest that one or more Ia unrestricted, labile and/or cell-associated factors, not active in most conventional T cell supernatants, are necessary to stimulate proliferation of small B cells.