Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Harmonic Mean01:09

Harmonic Mean

3.8K
The arithmetic mean is usually skewed towards the larger values in the data set. Therefore, to avoid this inherent bias towards smaller values, the harmonic mean is used.
Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
3.8K
Generator Voltage Control01:21

Generator Voltage Control

675
Generator voltage control is crucial for maintaining the stable operation of synchronous generators and wind turbines. In older models, a DC generator driven by the rotor delivers DC power to the rotor's field winding, and the power is transferred through slip rings and brushes. In the latest models, static or brushless exciters are used. Static exciters rectify AC power from the generator terminals and then transfer the DC power directly to the rotor. Brushless exciters, on the other hand, use...
675
Group Polarization01:01

Group Polarization

39.2K
Group polarization is the strengthening of an original group attitude following the discussion of views within a group (Teger & Pruitt, 1967). That is, if a group initially favors a viewpoint, after discussion the group consensus is likely a stronger endorsement of the viewpoint. Conversely, if the group was initially opposed to a viewpoint, group discussion would likely lead to stronger opposition.
39.2K
Glassware Calibration01:11

Glassware Calibration

1.5K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
1.5K
Molecular Shape and Polarity03:37

Molecular Shape and Polarity

76.0K
Dipole Moment of a Molecule
76.0K
Instrument Calibration01:12

Instrument Calibration

785
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
785

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The zonal evolution of collagen-network morphology quantified in early osteoarthritic grades of human cartilage.

Osteoarthritis and cartilage open·2022
Same author

Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography.

Scientific reports·2021
Same author

Analyzing the feasibility of discriminating between collagen types I and II using polarization-resolved second harmonic generation.

Journal of biophotonics·2018
Same author

Characterization of cellular and matrix alterations in the early pathogenesis of osteochondritis dissecans in pigs using second harmonic generation and two-photon excitation fluorescence microscopy.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2018
Same author

Comparison of Compressive Stress-Relaxation Behavior in Osteoarthritic (ICRS Graded) Human Articular Cartilage.

International journal of molecular sciences·2018
Same author

Non-linear optical microscopy of cartilage canals in the distal femur of young pigs may reveal the cause of articular osteochondrosis.

BMC veterinary research·2017

Related Experiment Video

Updated: Feb 12, 2026

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser
09:00

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser

Published on: June 28, 2018

10.5K

Automated calibration and control for polarization-resolved second harmonic generation on commercial microscopes.

Elisabeth I Romijn1, Andreas Finnøy1, Rajesh Kumar1

  • 1Department of Physics, Norwegian University of Science and Technology, Trondheim, Norway.

Plos One
|April 11, 2018
PubMed
Summary

Polarization-resolved second harmonic generation (P-SHG) microscopy can now be easily integrated into commercial microscopes. This automated system simplifies calibration and enhances accuracy for analyzing biological structures.

More Related Videos

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
14:18

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

Published on: February 28, 2016

11.9K
Harmonic Nanoparticles for Regenerative Research
09:23

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

12.2K

Related Experiment Videos

Last Updated: Feb 12, 2026

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser
09:00

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser

Published on: June 28, 2018

10.5K
Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
14:18

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

Published on: February 28, 2016

11.9K
Harmonic Nanoparticles for Regenerative Research
09:23

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

12.2K

Area of Science:

  • Biophysics
  • Microscopy
  • Biomedical Imaging

Background:

  • Polarization-resolved second harmonic generation (P-SHG) microscopy offers subresolution insights into biological macromolecular structure and orientation.
  • Wider adoption requires seamless integration with commercial laser scanning microscopes, alongside automated calibration and accuracy assessment.

Purpose of the Study:

  • To present an automated P-SHG setup easily integrated onto commercial multiphoton microscopes.
  • To enable rapid calibration and automated imaging for various polarization states.
  • To quantify measurement accuracy and system-dependent calibration settings.

Main Methods:

  • Developed a P-SHG setup for commercial multiphoton microscopes.
  • Implemented a fully automated system for calibration and imaging.
  • Included automated linear and circular polarization measurements of the excitation beam.
  • Utilized freely available software for calibration.

Main Results:

  • Demonstrated easy integration of the P-SHG setup onto commercial microscopes.
  • Showcased automated calibration and imaging capabilities.
  • Highlighted that calibration settings are highly system-dependent.
  • Quantified polarization control accuracy, showing it varies between systems.
  • Visualized result accuracy in real sample images.

Conclusions:

  • The automated P-SHG system facilitates rapid calibration and accurate imaging.
  • System-dependent calibration and polarization accuracy can be quantified and improved.
  • This development is expected to increase the adoption of P-SHG in biomedical applications.