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Updated: Feb 12, 2026

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
Published on: July 27, 2016
Immunochemical studies on native and glycated LDL - An approach to uncover the structural perturbations
Mohd Yasir Khan1, Sultan Alouffi2, Saheem Ahmad1
1IIRC-1, Laboratory of Glycation Biology and Metabolic Disorders, Integral University, Lucknow, India; Department of Biosciences, Integral University, Lucknow, India.
Abstract:
Glycation initiates with the non-enzymatic reaction of amino group of proteins and lipoproteins by carbonyl group of sugar moiety and intermediates of glycative stress such as methylglyoxal (MG). The initial glycation leads to the formation of early glycation products (Amadori products) which undergo rearrangement, cyclization and dehydration to form advanced glycation end products (AGEs). The main objective of the present study is to investigate the non-enzymatic glycation of low density lipoprotein (LDL) by MG at different concentration and at increasing incubation time period in vitro. This modification may increase the formation of Amadori products and AGEs which are physico-chemically characterized with respect to the extent of LDL modification. Additionally, immunogenicity of native and MG modified LDL (MG-LDL) was probed in female rabbits in vivo. Immunogenicity of MG-LDL was found to be highly immunogenic, eliciting high titer immunogen-specific antibodies while native form of LDL is less immunogenic. Furthermore, the histopathology and immune-fluorescence studies suggest that the kidney section of immunized rabbits exhibit the presence of immune complex (MG-LDL-IgG) deposition in the glomerular basement membrane (GBM).
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