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Updated: Feb 12, 2026

Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control
Published on: March 15, 2024
Analysis of two quorum sensing-deficient isolates of Pseudomonas aeruginosa
Dong Ju Lee1, A Ra Jo2, Min Cheol Jang2
1School of Biological Sciences and Technology, Chonnam National University, 77 Youngbong-ro, Buk-gu, Gwangju 61186, Republic of Korea; Institute of Basic Sciences, Chonnam National University, 77 Youngbong-ro, Buk-gu, Gwangju 61186, Republic of Korea.
Abstract:
Three strains of Pseudomonas aeruginosa were isolated: wild-type (WT, NO4) showed normal quorum sensing (QS), whereas QSD3 and QSD7 were QS-deficient (QSD) containing limited N-butyryl homoserine lactone (C4-HSL). The autoinducer activity produced by NO4 was found to be at least 50-fold higher than those by the QSD3 and the QSD7 strains. The QSDs produced lower levels of phenazine compounds (pyocyanin), siderophores (pyoverdine) and biosurfactants (rhamnolipids) than NO4. Therefore, the swarming motility and the swimming motility of the QSD3 and the QSD7 strains also decreased. Treatment with exogenous C4-HSL completely restored rhamnolipid production in both QSDs, suggesting that the biosynthesis of C4-HSL is defective. However, the biofilm production of the QSDs reached much higher levels than those of wild-types (NO4 and P. aeruginosa PAO1). And both QSD strains were more resistant than wild-type cell (NO4) against kanamycin and tobramycin. The RpoS gene, which function is related with QS, is point-nonsense mutated in QSD3 strain. But eleven QS-related genes in QSD3 were not mutated, compared to those of PAO1, which carries intact QS genes and is used as a positive control. This study is helpful in the development of novel approaches in the treatment of P. aeruginosa infections.
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