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Updated: Feb 12, 2026

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
Published on: July 18, 2014
Horseradish peroxidase-nanoclay hybrid particles of high functional and colloidal stability
Marko Pavlovic1, Paul Rouster2, Zoltan Somosi3
1Department of Inorganic and Analytical Chemistry, University of Geneva, CH-1205 Geneva, Switzerland.
Abstract:
Highly stable dispersions of enzyme-clay nanohybrids of excellent horseradish peroxidase activity were developed. Layered double hydroxide nanoclay was synthesized and functionalized with heparin polyelectrolyte to immobilize the horseradish peroxidase enzyme. The formation of a saturated heparin layer on the platelets led to charge inversion of the positively charged bare nanoclay and to highly stable aqueous dispersions. Great affinity of the enzyme to the surface modified platelets resulted in strong horseradish peroxidase adsorption through electrostatic and hydrophobic interactions as well as hydrogen bonding network and prevented enzyme leakage from the obtained material. The enzyme kept its functional integrity upon immobilization and showed excellent activity in decomposition of hydrogen peroxide and oxidation of an aromatic compound in the test reactions. In addition, remarkable long term functional stability of the enzyme-nanoclay hybrid was observed making the developed colloidal system a promising antioxidant candidate in biomedical treatments and industrial processes.
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