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Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
Published on: June 2, 2011
Highly sensitive label-free amperometric immunoassay of prostate specific antigen using hollow dendritic AuPtAg
Ya-Cheng Shi1, Ai-Jun Wang1, Pei-Xin Yuan1
1Key laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Abstract:
Herein, well-defined hollow dendritic AuPtAg alloyed nanocrystals (ANCs) were synthesized by a simple L-proline-mediated one-pot aqueous method. More importantly, the synthesized hollow dendritic architectures provide a suitable platform for immobilization of anti-prostate specific antigen (PSA). The resultant label-free immunosensor exhibited the improved performance for highly sensitive detection of PSA based on the enhanced catalytic currents of K3[Fe(CN)6] as a signal probe. Impressively, the immunosensor showed the wide linear range of 0.05-50 ng mL-1 and low detection limit of 0.017 ng mL-1 under optimal conditions for the assay of PSA, couple with the improved stability, reproducibility and selectivity. It provides a promising platform for clinical research and diagnosis.
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