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Updated: Feb 12, 2026

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
Published on: September 29, 2014
3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations
Leonid Andronov1,2,3,4,5, Jonathan Michalon1,2,3,4,5, Khalid Ouararhni1,2,3,4,5
1Centre for Integrative Biology (CBI), Department of Integrated Structural Biology, IGBMC, CNRS, Inserm, Université de Strasbourg, 1 rue Laurent Fries, Illkirch, France.
Motivation:
Single-molecule localization microscopy (SMLM) can play an important role in integrated structural biology approaches to identify, localize and determine the 3D structure of cellular structures. While many tools exist for the 3D analysis and visualization of crystal or cryo-EM structures little exists for 3D SMLM data, which can provide unique insights but are particularly challenging to analyze in three dimensions especially in a dense cellular context.
Results:
We developed 3DClusterViSu, a method based on 3D Voronoi tessellations that allows local density estimation, segmentation and quantification of 3D SMLM data and visualization of protein clusters within a 3D tool. We show its robust performance on microtubules and histone proteins H2B and CENP-A with distinct spatial distributions. 3DClusterViSu will favor multi-scale and multi-resolution synergies to allow integrating molecular and cellular levels in the analysis of macromolecular complexes.
Availability And Impementation:
3DClusterViSu is available under http://cbi-dev.igbmc.fr/cbi/voronoi3D.
Supplementary Information:
Supplementary figures are available at Bioinformatics online.
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