Related Experiment Video
Updated: Feb 12, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
Published on: November 15, 2021
Autofluorescence Imaging for Evaluating Debridement in Military and Trauma Wounds
Emily Blumenthal1, Steven Jeffery2
1University College London, Division of Surgery & Interventional Science, Gower Street, Bloomsbury, London, WC1E 6BT, UK.
Background:
Mechanisms for military injury have evolved in the past century. Debridement is the gold standard for preparing a clean wound bed, decreasing the bacterial load, and reducing the likelihood of infection. However, bacteria may continue to linger in these wounds. The MolecuLight i:X Imaging Device uses the principle of autofluorescence to detect bacteria under violet light. Thus, visualizing bacteria will not only guide clinicians in their management of the wound but it will also serve as a means of evaluating debridement efforts. We hope to improve traumatic wound management by targeting debridement and assessing its quality.
Methods:
We describe the use of the MolecuLight i:X to photograph wounds under standard and violet light in three patients. Images were captured before and after debridement. Microbiology swabs were collected to correlate the bacteria found in the images to the wounds.
Results:
The post-debridement images demonstrate a marked decrease or complete removal of bacteria. The microbiology swabs confirmed the pre-debridement presence of bacteria.
Conclusions:
The MolecuLight i:X shows promise for debridement evaluation. The use of the MolecuLight i:X may reduce the likelihood of infection, thus having positive implications for military and trauma wounds.
Related Concept Videos
Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Self-Evaluation: Self-Enhancement and Self-Verification
Nursing Evaluation
Self-Evaluation Maintenance Model
Dysrhythmias V: Evaluating Dysrhythmias
Social Foundations of Self III: Self-Evaluation

