Related Experiment Video

Updated: Feb 12, 2026

A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
05:06

A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis

Published on: October 24, 2015

12.9K

Pterostilbene inhibits MTA1/HDAC1 complex leading to PTEN acetylation in hepatocellular carcinoma

Yu-Yuan Qian1, Zhi-Su Liu1, Hua-Jun Yan2

  • 1Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|April 12, 2018
PubMed
Abstract

Insights

Pterostilbene (PTER) inhibits hepatocellular carcinoma (HCC) growth by downregulating Metastasis-Associated Protein 1 (MTA1) and histone deacetylase 1 (HDAC1), promoting cancer cell apoptosis and reducing invasion.

Area of Science:

  • Oncology
  • Epigenetics
  • Molecular Biology

Background:

  • Hepatocellular carcinoma (HCC) remains a significant global health challenge.
  • Metastasis-Associated Protein 1 (MTA1) and histone deacetylase 1 (HDAC1) complex play crucial roles in HCC progression.
  • Targeting epigenetic regulators offers a promising therapeutic strategy for HCC.

Purpose of the Study:

  • To investigate the inhibitory effects of pterostilbene (PTER) on HCC growth.
  • To elucidate the role of PTER in modulating the MTA1/HDAC1 complex.
  • To determine the impact of PTER on PTEN acetylation and its downstream effects in HCC.

Main Methods:

  • Cell culture of SMMC-7721 hepatoma cells and treatment with PTER.
  • Western blot analysis for protein expression and real-time PCR for MTA1 mRNA.
  • Immunoprecipitation to assess PTEN acetylation.
  • Establishment of animal models to validate anti-cancer effects.

Main Results:

  • PTER treatment downregulated MTA1 and HDAC1 expression in HCC cells.
  • PTER destabilized the MTA1/HDAC1 complex, leading to increased PTEN acetylation at Lys402.
  • Downregulation of MTA1 correlated with increased apoptosis and reduced invasion in HCC.

Conclusions:

  • Pterostilbene effectively suppresses HCC growth and invasion by targeting the MTA1/HDAC1/NuRD complex.
  • PTER promotes PTEN acetylation and apoptosis, offering a novel epigenetic approach for HCC treatment.
  • These findings highlight PTER's potential in chemoprevention and as an anticancer/antimetastatic therapeutic agent.
Keywords:
Ac-PTENHCCHDAC1MTA1PTENPterostilbene

More Related Videos

Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients05:31

Transradial Access Chemoembolization for Hepatocellular Carcinoma Patients

Published on: September 20, 2020

6.2K
Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice10:35

Generation of Subcutaneous and Intrahepatic Human Hepatocellular Carcinoma Xenografts in Immunodeficient Mice

Published on: September 25, 2013

37.2K

Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.3K
Phase II Reactions: Acetylation Reactions01:24

Phase II Reactions: Acetylation Reactions

Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
833
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Phase-lead and Phase-lag Controllers01:22

Phase-lead and Phase-lag Controllers

Understanding the working function of different types of controllers can be illustrated with practical analogies, such as adjusting a stereo's volume equalizer. Cranking up the bass involves a phase-lead controller, which functions as a high-pass filter, while increasing the treble uses a phase-lag controller, which acts as a low-pass filter. PD controllers, similar to high-pass filters, enhance the system's response to high-frequency components. PI controllers, akin to low-pass...
582
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.8K
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K