Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.6K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.4K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.4K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.1K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.1K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

9.7K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
9.7K
Peroxisomes01:24

Peroxisomes

19.8K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
19.8K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

16.8K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
16.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Effectiveness, Safety and Quality of Life With Ustekinumab for the Treatment of Crohn's Disease: Results of the UK Ustekinumab Real World Evidence Study (UndieS) Study Group.

Alimentary pharmacology & therapeutics·2026
Same author

Deuterated Pentoxifylline Analog in the Treatment of Necrobiosis Lipoidica: A Nonrandomized Clinical Trial.

JAMA dermatology·2026
Same author

Rural-urban differences in the perception of cooperation between schools and the psychiatric care system for children and adolescents, and attitudes towards the ban on using phones at school - a cross-sectional study.

Annals of agricultural and environmental medicine : AAEM·2026
Same author

In Vivo Emergence of Oxacillin Resistance in mecA-Negative MSSA Endocarditis With Metastatic Infection.

Clinical case reports·2026
Same author

Determination of Trace Nitrosamines in Plastic Pharmaceutical Packaging Materials.

PDA journal of pharmaceutical science and technology·2026
Same author

Literature horizon scan for new scientific data on plants, microorganisms and animals, and their products obtained by new genomic techniques (October 2025).

EFSA journal. European Food Safety Authority·2026

Related Experiment Video

Updated: Jan 1, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.8K

Protein Promiscuity in H2O2 Signaling.

David Young1,2,3, Brandan Pedre1,2,3, Daria Ezeriņa1,2,3

  • 11 Center for Structural Biology, VIB, Brussels, Belgium.

Antioxidants & Redox Signaling
|April 12, 2018
PubMed
Summary

Cellular responses to oxidative stress involve complex redox signaling pathways. Hydrogen peroxide (H2O2) signaling utilizes thiol peroxidases in a redox relay, influencing transcription factors and cell fate.

Keywords:
moonlighting functionalityperoxidase signalingthiol redoxtranscription factors

More Related Videos

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

12.0K
Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

15.6K

Related Experiment Videos

Last Updated: Jan 1, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

7.8K
Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

12.0K
Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
06:45

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay

Published on: May 26, 2011

15.6K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Understanding cellular responses to oxidative stress is crucial for deciphering redox signaling pathways.
  • Redox signaling encompasses oxidant sensing, protein function modulation, and transcriptional regulation.
  • Hydrogen peroxide (H2O2) signaling is increasingly understood through a thiol peroxidase redox relay mechanism.

Purpose of the Study:

  • To elucidate the mechanisms of cellular response to oxidative stress.
  • To detail the role of hydrogen peroxide (H2O2) in redox signaling.
  • To explore the regulation of transcriptional processes and cell fate under oxidative conditions.

Main Methods:

  • Review of current literature on redox signaling pathways.
  • Analysis of the thiol peroxidase redox relay model for H2O2 signaling.
  • Examination of protein-protein interactions in redox-regulated networks.

Main Results:

  • Thiol peroxidases act as upstream sensors for H2O2, initiating redox relays.
  • Redox signaling is complicated by moonlighting proteins with promiscuous interactions.
  • Mammalian systems employ kinase-dependent and direct oxidative pathways for transcription factor regulation.

Conclusions:

  • Transcriptional regulation and cell fate are modulated by oxidative regulation of kinase pathways and redox-dependent protein associations.
  • Peroxiredoxins (Prxs) and redox-responsive moonlighting proteins play key roles in H2O2-mediated signaling networks.
  • Complex cross-regulatory networks with multiple oxidative regulation nodes govern cellular responses to H2O2.