L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity

Acrylamide is a widespread substance having many areas of utilization. Besides, it also forms a part of high-temperature processed starchy foods. To date, numerous in vivo and in vitro studies have documented that acrylamide has toxic effects on various organ systems.

To determine the potential protective effect of L-cysteine on acrylamide-induced testicular toxicity.

Animal experimentation.

We randomly divided 28 rats into four groups as control (0.9% saline), L-cysteine (150 mg/kg), acrylamide (40 mg/kg) and acrylamide + L-cysteine. After a 10-day intraperitoneal injection period, we euthanized the animals, recorded their body and testis weights, collected blood samples for serum testosterone measurement, and excised testes for histopathological and morphometric evaluation. Besides, immunohistochemical scoring of proliferating cell nuclear antigen and bax proteins was performed.

Acrylamide reduced body (p<0.01) and testis weights (p<0.05), seminiferous tubule diameter (p<0.001) and proliferating cell nuclear antigen expression (p<0.05), while it increased bax protein expression (p<0.01) and the percentage of seminiferous tubules that contain multinucleated giant cells (p<0.001), but did not significantly change serum testosterone levels when compared to control. L-cysteine administered with acrylamide decreased multinucleated giant cell number (p<0.001) and reversed the reduced proliferating cell nuclear antigen positivity (p<0.001), but did not restore other parameters compared with the acrylamide alone-treated group.

Taking into account the dose and duration employed, the present study concluded that L-cysteine partially protects testis against acrylamide-induced toxic effects.