Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Heat and Free Expansion01:24

Heat and Free Expansion

3.0K
The work done by a thermodynamic system depends not only on the initial and final states but also on the intermediate states—that is, on the path. Like work, when heat is added to a thermodynamic system, it undergoes a change of state, and the state attained depends on the path from the initial state to the final state. Consider an ideal gas cylinder fitted with a piston. When the cylinder is heated at a constant temperature, the gas molecules absorb energy and expand slowly in a...
3.0K
Thermal Expansion01:22

Thermal Expansion

5.7K
The expansion of alcohol in a thermometer is one of many commonly encountered examples of thermal expansion, which is the change in size or volume of a given system as its temperature changes. The most visible example is the expansion of hot air. When air is heated, it expands and becomes less dense than the surrounding air, which then exerts an upward force on the hot air to, for example, make steam and smoke rise, and hot air balloons float. The same behavior happens in all liquids and gases,...
5.7K
Cell Culture01:21

Cell Culture

22.9K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
22.9K
Binomial Expansion Using Pascal's Triangle01:30

Binomial Expansion Using Pascal's Triangle

270
Expanding a binomial expression such as (a + b)n results in a predictable sequence of terms that can be systematically derived using Pascal’s Triangle. This triangular array of numbers plays a central role in understanding and computing the coefficients of binomial expansions.Pascal’s Triangle is constructed such that each row corresponds to the coefficients of a binomial raised to a power. The topmost row, known as the zeroth row, corresponds to (a + b)0, and each successive row...
270
Expansion and Contraction in Masonry Walls01:19

Expansion and Contraction in Masonry Walls

1.4K
Masonry walls are subject to slight expansion and contraction due to variations in temperature and moisture. Thermal movement in masonry is relatively straightforward to measure and plan for. On the other hand, moisture movement poses more of a challenge. New clay masonry units typically absorb water and expand over time under normal environmental conditions. Conversely, new concrete masonry units tend to shrink as they lose the excess moisture acquired during their production process.
To...
1.4K
Stem Cell Culture01:17

Stem Cell Culture

6.1K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A precision gene-engineered B cell medicine producing sustained levels of active factor IX for hemophilia B therapy.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Using Metabolomics to Identify Cell Line-Independent Indicators of Growth Inhibition for Chinese Hamster Ovary Cell-based Bioprocesses.

Metabolites·2020
Same author

The importance and future of biochemical engineering.

Biotechnology and bioengineering·2020
Same author

Multi-Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing.

Biotechnology journal·2020
Same author

CHO genome mining for synthetic promoter design.

Journal of biotechnology·2019
Same author

Multi-Omics Study on the Impact of Cysteine Feed Level on Cell Viability and mAb Production in a CHO Bioprocess.

Biotechnology journal·2018

Related Experiment Video

Updated: Feb 12, 2026

Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product
11:51

Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product

Published on: September 22, 2021

9.9K

Innovation in Cell Banking, Expansion, and Production Culture.

Rashmi Kshirsagar1, Thomas Ryll2

  • 1Technical Development, Biogen, 225 Binney Street, Cambridge, MA, 02142, USA.

Advances in Biochemical Engineering/Biotechnology
|April 12, 2018
PubMed
Summary

Innovations in cell culture processes enhance recombinant protein production. Key improvements in cell banking, expansion, and production cultures increase efficiency, robustness, and safety for biopharmaceutical manufacturing.

Keywords:
Cell banking, Fed-batch, Innovation, Mammalian cell culture, Productivity

More Related Videos

Isolation and Culture Expansion of Tumor-specific Endothelial Cells
10:15

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

11.7K
The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups
14:14

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

Published on: May 13, 2022

6.4K

Related Experiment Videos

Last Updated: Feb 12, 2026

Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product
11:51

Expansion and Enrichment of Gamma-Delta γδ T Cells from Apheresed Human Product

Published on: September 22, 2021

9.9K
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
10:15

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

11.7K
The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups
14:14

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

Published on: May 13, 2022

6.4K

Area of Science:

  • Biotechnology
  • Biopharmaceutical Manufacturing
  • Cell Culture Technology

Background:

  • Cell culture processes are essential for producing recombinant protein therapeutics.
  • These processes involve critical steps: cell banking, seed expansion, and production culture.
  • Optimizing these steps is key to achieving high product yield and quality.

Purpose of the Study:

  • To review significant innovations in cell culture process elements.
  • To highlight improvements in efficiency, robustness, and safety of recombinant protein production.
  • To provide an overview of advancements in biopharmaceutical manufacturing.

Main Methods:

  • Review of innovations in cell bank storage and thawing.
  • Analysis of seed expansion techniques for sufficient cell mass.
  • Examination of production culture optimization strategies for yield and quality.

Main Results:

  • Identified key innovations across all stages of cell culture production.
  • Demonstrated improvements in process efficiency and robustness.
  • Showcased enhanced product quality and safety profiles.

Conclusions:

  • Recent innovations have significantly advanced cell culture-based recombinant protein production.
  • These advancements lead to more efficient, robust, and safer biopharmaceutical manufacturing processes.
  • Continuous innovation is crucial for meeting the growing demand for protein therapeutics.